Through the collaboration of its partners and experts, CitiesWithNature has developed a range of Guides that provide helpful, science-based information on numerous topics relevant to cities. Guides are essentially small, digestible amounts of technical information on different themes aimed at making the information interactive and user-friendly. Based on best practices, the Guides provide practical and simple step-by-step guidance to support cities to take action. In particular, the Guides aim to empower cities with the required knowledge about different areas of expertise to be able to make commitments on the Convention on Biological Diversity-recognized CitiesWithNature Action Platform.